Quantitative Phase Imaging in optical (super-resolution) microscopy for biology studies
Par Pierre Bon, Laboratoire Photonique, Numérique et Nanosciences (LP2N), CNRS-IOGS-UB
Mardi 12 Janvier, 14h, Salle des séminaires, 3ème étage, Batiment A4
Abstract :
Quantitative phase imaging (QPI) is a powerful optical method to enhance the knowledge about a micro/nanoscopic samples. It relies on the capability to measure not only the intensity of the light but also its phase. This provides several types of information depending on the illumination scheme or imaging mode.
QPI was initially developed to image microscopic structures inside semi-transparent samples (ex. nucleus), without labelling/staining and using coherent transmitted light -such as a laser. I will show recent breakthrough using our QPI technique based on quadriwave lateral shearing interferometry [1] coupled with incoherent halogen trans-illumination. It allows label-free cytoskeleton and organelle trafficking imaging at up to 50 Hz and for any duration [2].
I will then present for the capability to retrieve the 3D spatial distribution of particles (such as gold nanobeads) with a sub-nanometric localization by the simultaneous use of intensity and phase imaging. I will show the use of this method to stabilize the drift of a super-resolution microscope.
[1] Bon, Maucort, Wattellier, and Monneret, “Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells,” Opt. Express 17, 13080-13094 (2009)
[2] Bon, Lécart, Fort, Lévêque-Fort, “Fast Label-Free Cytoskeletal Network Imaging in Living Mammalian Cells”, Biophysical J., 106, 1588 – 1595 (2014)
[3] Bon, Bourg, Lécart, Monneret, Fort, Wenger, Lévêque-Fort, “Three-dimensional nanometre localization of nanoparticles to enhance super-resolution microscopy”, Nat. Comm.,6 , 7764 (2015)