A phase diagram for the organization of cell colonies
Par Romaric Vincent, CEA, Grenoble
Mardi 28 Novembre, 14h, Salle des séminaires (215), 2ème étage, Bâtiment A4N
The regular distribution of neuronal cells, self-healing epithelial monolayers or 3D tumor formation are examples of active-organization of cells in tissues. While cell-substrate and cell-cell adhesions have a widely acknowledged implication in these macroscopic organizations, the role of the cell-specific contact repulsion called contact inhibition of locomotion (CIL) is less clear. In this work, we include these fundamental cellular functions in large scale 2D simulations of cell-like particles. We built a phase diagram which describes within the same unifying framework several of the known multicellular tissues. We provide theoretical scaling laws for transitions such as epithelial-mesenchymal transitions or the dewetting of a 2D epithelium into a 3D agglomerate, which signpost the biophysical pathways available to control these transitions. At last, I will present preliminary results of lens-free microscopy, a method that offers a unique mean to test the organization of large cell colonies.