Infrared surface plasmon and waveguide modes spectroscopy for live cell biosensing
Par Victor Yashunsky, Laboratoire Physico-Chimie Curie, Institut Curie, Paris
Mardi 24 Mai, 14h, Salle des séminaires, 3ème étage, Batiment A4
Abstract :
In addition to surface plasmon resonance in infrared range, we discovered a novel bio-optical phenomenon of waveguide modes propagating inside living cells. We found that such waveguide modes can be observed in intact epithelial cell monolayers and thus can provide complimentary information about cell connectivity and cell layer thickness.
Combined together surface plasmon and waveguide modes enable precise structural analysis of cell populations in real-time and in label-free manner. This has great importance for understanding the collective behavior of cells in the context of tissues and organs.
Reférences :
1. Yashunsky, V. et al. Real-Time Sensing of Enteropathogenic E. coli-Induced Effects on Epithelial Host Cell Height, Cell-Substrate Interactions, and Endocytic Processes by Infrared Surface Plasmon Spectroscopy . PloS ONE 8, e78431 (2013).
2. Yashunsky, V. et al. Surface plasmon-based infrared spectroscopy for cell biosensing. Journal of Biomedical Optics 17, 081409-1-081409-8 (2012).
3. Yashunsky, V. et al. Real-Time Sensing of Cell Morphology by Infrared Waveguide Spectroscopy. PloS ONE 7, e48454 (2012).
4. Yashunsky, V., Lirtsman, V., Golosovsky, M., Davidov, D. & Aroeti, B. Real-time monitoring of epithelial cellcell and cell-substrate interactions by infrared surface plasmon spectroscopy. Biophysical Journal 99, 4028-4036 (2010).
5. Yashunsky, V. et al. Real-time monitoring of transferrin-induced endocytic vesicle formation by mid-infrared surface plasmon resonance. Biophysical Journal 97, 1003-1012 (2009).